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Tutorial Intro

Let's discover Docusaurus in less than 5 minutes.

Getting Started​

Get started by creating a new site.

Or try Docusaurus immediately with docusaurus.new.

What you'll need​

  • Node.js version 18.0 or above:
    • When installing Node.js, you are recommended to check all checkboxes related to dependencies.

Generate a new app​

Generate a new Docusaurus site using the classic template.

The classic template will automatically be added to your project after you run the command:

npm init docusaurus@latest my-website classic

You can type this command into Command Prompt, Powershell, Terminal, or any other integrated terminal of your code editor.

The command also installs all necessary dependencies you need to run Docusaurus.

Start your site​

Run the development server:

cd my-website
npm run start

The cd command changes the directory you're working with. In order to work with your newly created Docusaurus site, you'll need to navigate the terminal there.

The npm run start command builds your website locally and serves it through a development server, ready for you to view at http://localhost:3000/.

Open docs/intro.md (this page) and edit some lines: the site reloads automatically and displays your change

Sample information​

⬝Name: (sample name)

⬝Type: ( choose 1 of 4 type)

-Pacbio is a biotech company in USA. One of PacBio's famous products is the SMRT system which is currently widely used in molecular biology and genetics research to analyze gene sequences and gene structures.

  • SMRT sequencing is a type of long-read sequencing. In SMRT sequencing, DNA sequences are read longer than in short-read sequencing. Specifically, readable sequence fragments can reach tens or even hundreds of thousands of bp each, helping to identify complex gene structures and detect major gene variations. Therefore, PacBio's technology is a long-read sequencing method, which offers advantages in capturing complex genetic details and detecting large gene variants.

-Nanopore sequencing is a DNA and RNA sequencing method, based on the use of nanotek techniques to determine the sequence of nucleic molecules. This technology is part of third-generation sequencing (NGS), with the main advantage being the ability to directly sequence nucleic molecules without the need to copy into multiple copies like traditional sequencing methods.

  • Nanopore sequencing technology offers a number of advantages, including direct sequencing, fast speeds, the ability to process large samples, and the ability to operate on a variety of sample types. In nanopore sequencing, each strand of DNA or RNA is read through a small hole (nanopore), and through tracking changes in electrical current, the nucleotides are identified sequentially.

  • Due to the nature of this process, nanopore sequencing allows long and continuous sequences to be read without having to break them down into fragments as in short-read sequencing. Therefore, nanopore sequencing technology is often considered long-read sequencing, with the ability to read strings with lengths that can reach tens or even hundreds of thousands of bp.

-Dbnseq: DNBSEQ™ technology is BGI's proprietary sequencing technology that reduces the error rate that occurs during amplification to EXTREMELY LOW levels thanks to the Rolling Circle Amplification (RCA) technique.

  • DNBSEQ™ creates DNA NanoBalls (DNBs) combined with the use of Flow Cell chips with extremely sophisticated design, high density to help the activation sites are distributed evenly, avoiding interference with fluorescent signals. At the moment, DNBSEQ™ is the most optimal solution compared to existing sequencing platforms.

  • Sequencing technology by "probe-anchor complex" (cPAS). Thanks to the improved cohesion of cPAS, it increases the accuracy in sequencing with greater sequencing reading speed and length. The Biochemistry Research and Development Division (BGI) has studied a vast range of reaction conditions and screened tens of thousands of sequencing enzymes to help complete biochemical reactions in just 60 seconds. Advanced image processing software uses sub-pixel algorithms and multi-threaded parallel compression to analyze images in real-time and accurately read bases with market-leading data processing speeds.

  • DNBSEQ's™ library construction method does not use traditional PCR techniques like other platforms on the market, so it has higher accuracy in detecting SNP and InDel mutations. In addition, the index jump rate in BGI's sequencing platforms (BGISEQ/MGISEQ) is much lower than in other platforms.

-Illumina is one of the leading companies in the field of sequencing and gene analysis. The main technology of Illumina company is short-read sequencing. Illumina's sequencing technology uses amplification and cluster amplification to create a large number of clusters of DNA fragments in a single reaction. The fragments are then sequenced simultaneously using single nucleotides used to identify each location on the chain.

  • Illumina's sequencing technology provides high performance, good accuracy and affordable cost compared to previous sequencing methods. It has opened up many new opportunities in genetic research, molecular biology, personalized medicine and many other fields.

⬝Project: (choose project) ➔Upload samples

Type: Pacbio​